complexiolyte cl- 91 Search Results


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cells recombinant dna reagent mouse cnnm3 myc flag  (OriGene)
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Figure 3. Heterologous expression of transient receptor potential melastatin-subfamily member 7 (TRPM7) in Xenopus oocytes. (A, B) Two-electrode voltage clamp (TEVC) measurements of TRPM7 currents. (A) Left panel: Representative current-voltage (I-V) relationships of TRPM7 currents measured in oocytes expressing TRPM7 alone or TRPM7 with <t>CNNM3</t> or ADP-ribosylation factor-like protein 15 (ARL15) (cRNAs ratio 2:1), and TRPM7 with CNNM3 and ARL15 (cRNAs ratio 2:1:1). Right panel: Current amplitudes (mean ± standard error of the mean [SEM]) at +80 mV in measurements shown on the
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Figure 3. Heterologous expression of transient receptor potential melastatin-subfamily member 7 (TRPM7) in Xenopus oocytes. (A, B) Two-electrode voltage clamp (TEVC) measurements of TRPM7 currents. (A) Left panel: Representative current-voltage (I-V) relationships of TRPM7 currents measured in oocytes expressing TRPM7 alone or TRPM7 with <t>CNNM3</t> or ADP-ribosylation factor-like protein 15 (ARL15) (cRNAs ratio 2:1), and TRPM7 with CNNM3 and ARL15 (cRNAs ratio 2:1:1). Right panel: Current amplitudes (mean ± standard error of the mean [SEM]) at +80 mV in measurements shown on the
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Image Search Results


Journal: eLife

Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics

doi: 10.7554/eLife.68544

Figure Lengend Snippet:

Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

Techniques: Stable Transfection, Expressing, Affinity Purification, Recombinant, Plasmid Preparation, Protein Concentration, Sequencing, Modification, Software

Figure 3. Heterologous expression of transient receptor potential melastatin-subfamily member 7 (TRPM7) in Xenopus oocytes. (A, B) Two-electrode voltage clamp (TEVC) measurements of TRPM7 currents. (A) Left panel: Representative current-voltage (I-V) relationships of TRPM7 currents measured in oocytes expressing TRPM7 alone or TRPM7 with CNNM3 or ADP-ribosylation factor-like protein 15 (ARL15) (cRNAs ratio 2:1), and TRPM7 with CNNM3 and ARL15 (cRNAs ratio 2:1:1). Right panel: Current amplitudes (mean ± standard error of the mean [SEM]) at +80 mV in measurements shown on the

Journal: eLife

Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics

doi: 10.7554/elife.68544

Figure Lengend Snippet: Figure 3. Heterologous expression of transient receptor potential melastatin-subfamily member 7 (TRPM7) in Xenopus oocytes. (A, B) Two-electrode voltage clamp (TEVC) measurements of TRPM7 currents. (A) Left panel: Representative current-voltage (I-V) relationships of TRPM7 currents measured in oocytes expressing TRPM7 alone or TRPM7 with CNNM3 or ADP-ribosylation factor-like protein 15 (ARL15) (cRNAs ratio 2:1), and TRPM7 with CNNM3 and ARL15 (cRNAs ratio 2:1:1). Right panel: Current amplitudes (mean ± standard error of the mean [SEM]) at +80 mV in measurements shown on the

Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

Techniques: Expressing

Figure 4. Effects of ADP-ribosylation factor-like protein 15 (ARL15) and CNNM3 on Mg2+ currents of the transient receptor potential melastatin-subfamily member 7 (TRPM7) channel expressed in Xenopus oocytes. TEVC measurements were performed using the external ND96 solution containing 3 mM Mg2+and no other divalent cations. (A, B) Assessment of oocytes expressing TRPM7 or co-expressing TRPM7 with ARL15 (cRNA ratio

Journal: eLife

Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics

doi: 10.7554/elife.68544

Figure Lengend Snippet: Figure 4. Effects of ADP-ribosylation factor-like protein 15 (ARL15) and CNNM3 on Mg2+ currents of the transient receptor potential melastatin-subfamily member 7 (TRPM7) channel expressed in Xenopus oocytes. TEVC measurements were performed using the external ND96 solution containing 3 mM Mg2+and no other divalent cations. (A, B) Assessment of oocytes expressing TRPM7 or co-expressing TRPM7 with ARL15 (cRNA ratio

Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

Techniques: Expressing